Autoradiographic Localization of [3H]-Nisoxetine Binding Sites in the CNS of Male and Female Japanese Quail

Background In the central nervous system of mammals, transporters localized on the presynaptic nerve terminals regulate the reuptake of neurotransmitters. These transporters are selective for a specific neurotransmitter such as dopamine (DA) and norepinephrine (NE). Specifically in the synapse, the dopamine transporter (DAT) reuptakes DA and the norepinephrine transporter (NET) reuptakes NE. However previous research has found that avian species do not have a gene for DAT, and therefore, birds may be using the NET to clear both NE and DA from the synapse. The current study aimed to extend this finding by localizing NET expression in male and female Japanese quail (Coturnix japonica) brains using [3H]Nisoxetine, a selective NET blocker. Results High densities of binding sites were observed in the olfactory tubercle (OTu), the medial striatum (MSt), and the lateral striatum (LSt). Lower densities of binding sites were detected in the amygdala (AMY) and hypothalamus (Hyp), and low binding was found in the medial preoptic area (mPOA) and the pallium. Conclusion The areas with the highest densities of NET are also areas that previous research has shown to have high levels of DA activity but low levels of NE innervation (e.g. striatum). The distribution of this reuptake transporter is consistent with the theory that NET acts to clear both DA and NE from the synapse

Advanced diagnostic genetic testing in inherited retinal disease: experience from a single tertiary referral centre in the UK National Health Service

In 2013, as part of our genetic investigation of patients with inherited retinal disease, we utilized multigene panel testing of 105 genes known to cause retinal disease in our patient cohorts. This test was performed in a UK National Health Service (NHS) accredited laboratory. The results of all multigene panel tests requested between 1.4.13 and 31.8.14 were retrospectively reviewed. All patients had been previously seen at Moorfields Eye Hospital, London, UK and diagnosed with an inherited retinal dystrophy after clinical examination and detailed retinal imaging. The results were categorized into three groups: (i) Testing helped establish a certain molecular diagnosis in 45 out of 115 (39%). Variants in USH2A (n = 6) and RP1 (n = 4) were most common. (ii) Definitive conclusions could not be drawn from molecular testing alone in 13 out of 115 (11%) as either insufficient pathogenic variants were discovered or those identified were not consistent with the phenotype. (iii) Testing did not identify any pathogenic variants responsible for the phenotype in 57 out of 115 (50%). Multigene panel testing performed in an NHS setting has enabled a molecular diagnosis to be confidently made in 40% of cases. Novel variants accounted for 38% of all identified variants. Detailed retinal phenotyping helped the interpretation of specific variants. Additional care needs to be taken when assessing polymorphisms in genes that have been infrequently associated with disease, as historical techniques were not as rigorous as contemporary ones. Future iterations of sequencing are likely to offer higher sensitivity, testing a broader range of genes, more rapidly and at a reduced cost

Effects Of Leisure Education Programme On Leisure Benefits And Motivation Among Rural Iban Adolescents In Sibu, Sarawak

Introduction: Leisure education is becoming an important concept in the Western world, but less established and practiced in Malaysia. Evidence has shown the positive effects of leisure education programme to reduce adolescent’s engagement in risky behaviours, such as substance use, sexual behaviour, obesity, and internet addiction, which are detrimental to adolescent’s health and well-being. This study was aimed to examine the effects of leisure education programme on leisure benefits and motivation aspects among rural Iban adolescents in Sibu, Sarawak. Methods: The study employed a pre-post evaluation design. Convenience sampling was used to select the participants.Thirty four Iban adolescents from two longhouses had completed 9-hour leisure education programme for three weeks. Respondents were surveyed before and immediately after the intervention to observe changes in knowledge of leisure benefits and free time motivation scales. Data were analyzed with the use of Wilcoxon signed ranks tests. Significance level was set at p<0.05. Results: The leisure education intervention showed improvement in respondent’s knowledge on benefits of leisure time, but no significant changes was detected in all five motivation types (intrinsic, amotivation, extrinsic, introjected and identified). Conclusion: The leisure education intervention conducted had effectively improved the knowledge of the respondents on leisure benefits. Therefore, leisure education service can be considered to promote a balanced and healthy leisure lifestyle among adolescents. Keywords: Adolescent, Knowledge, Leisure Education, Motivation, Rura

Agri-Info: Cloud Based Autonomic System for Delivering Agriculture as a Service

The Internet of Things (IoT) and cloud computing paradigms offer enhanced services for agricultural applications to manage the data efficiently. To provide an effective and reliable agriculture as a service, there is a need to manage Quality of Service (QoS) parameters to efficiently monitor and measure the delivered services dynamically. This paper presents a QoS-aware cloud based autonomic information system called Agri-Info for delivering agriculture related information as a service through the use of latest Internet-based technologies such as cloud computing and IoT which manage various types of agriculture related data based on different domains of agricultural industry. Proposed system gathers information from various users through preconfigured IoT devices (mobiles, laptops or iPads). It further manages and delivers the required information to users and diagnoses the agriculture status automatically. We have developed the web and mobile-based application and evaluated the performance of the proposed system in cloud environment using CloudSim toolkit based small scale environment. Results demonstrate our system yields in a reduction on 12.46% cost, on 15.52% network bandwidth, on 10.18% execution time and 13.32% in latency. Furthermore, a case study of an Indian village is presented to identify the customer satisfaction of farmers

The suitability of an uncemented hydroxyapatite coated (HAC) hip hemiarthroplasty stem for intra-capsular femoral neck fractures in osteoporotic elderly patients: the Metaphyseal-Diaphyseal index, a solution to preventing intra-operative periprosthetic fracture

This study will seek to identify a measurable radiographic index, the Metaphyseal-Diaphyseal Index (MDI) score to determine whether intra-operative fracture in osteoporotic bone can be predicted

Defective monocyte-derived macrophage phagocytosis is associated with exacerbation frequency in COPD

Background Lower airway bacterial colonisation (LABC) in COPD patients is associated with increased exacerbation frequency and faster lung function decline. Defective macrophage phagocytosis in COPD drives inflammation, but how defective macrophage function contributes to exacerbations is not clear. This study investigated the association between macrophage phagocytosis and exacerbation frequency, LABC and clinical parameters. Methods Monocyte-derived macrophages (MDM) were generated from 92 stable COPD patients, and at the onset of exacerbation in 39 patients. Macrophages were exposed to fluorescently labelled Haemophilus influenzae or Streptococcus pneumoniae for 4 h, then phagocytosis measured by fluorimetry and cytokine release by ELISA. Sputum bacterial colonisation was measured by PCR. Results Phagocytosis of H. influenzae was negatively correlated with exacerbation frequency (r = 0.440, p < 0.01), and was significantly reduced in frequent vs. infrequent exacerbators (1.9 × 103 RFU vs. 2.5 × 103 RFU, p < 0.01). There was no correlation for S. pneumoniae. There was no association between phagocytosis of either bacteria with age, lung function, smoking history or treatment with inhaled corticosteroids, or long-acting bronchodilators. Phagocytosis was not altered during an exacerbation, or in the 2 weeks post-exacerbation. In response to phagocytosis, MDM from exacerbating patients showed increased release of CXCL-8 (p < 0.001) and TNFα (p < 0.01) compared to stable state. Conclusion Impaired COPD macrophage phagocytosis of H. influenzae, but not S. pneumoniae is associated with exacerbation frequency, resulting in pro-inflammatory macrophages that may contribute to disease progression. Targeting these frequent exacerbators with drugs that improve macrophage phagocytosis may prove beneficial

Decoupling of a Current-Biased Intrinsic Josephson Junction from its Environment

We have observed a dissipative phase diffusion branch in arrays of hysteretic high-Tc intrinsic Josephson junctions. By comparing the data with a thermal activation model we extract the impedance seen by the junction in which phase diffusion is occurring. At the plasma frequency this junction is isolated from its environment and it sees its own large (~ kilo Ohm) impedance. Our results suggest that stacks of Josephson junctions may be used for isolation purposes in the development of a solid state quantum computer